Differences in the prognosis of HPV16-positive patients with squamous cell carcinoma of head and neck according to viral load and expression of P16

2018
journal article
article
17
cris.lastimport.wos2024-04-09T20:30:32Z
dc.abstract.enPurpose To evaluate the impact of HPV16 load (VL-the number of virus genome copies per cell) and P16 expression on prognosis of patients with squamous cell carcinomas (SCCs) of head and neck (HN). Materials and methods HPV16 presence was assessed in the group of 109 patients with HNSCCs by quantitative polymerase chain reaction (qPCR). VL (assessed by qPCR) and P16 expression (evaluated by immunohistochemistry) were analysed only in the subgroup of HPV16-positive tumours. These features were correlated with 5-year overall survival (OS) and disease-free survival (DFS). Results HPV16 infection was found in 36 tumours (33.0%). Virus-positive patients had better OS and DFS than those without infection (P = 0.041 and 0.005). Among HPV16-positive HNSCCs, 18 (50.0%) had higher VL (median value > 6764.3 copies/cell) and 25 (73.5%) P16 over expression. The significant differences in OS and DFS (P = 0.008 and 0.004) were noticed according to VL, wherein 100% DFS was found for patients with higher VL. According to P16 expression, significant difference was found only for OS (P = 0.020). In multivariate analysis, VL (P = 0.045; HR = 2.795; CI 0.121-1.060) and the level of smoking (P = 0.023, HR = 2.253; CI 1.124-4.514) were independent factors affecting DFS of HPV16-positive patients. Conclusion On the basis of viral load, it is possible to differentiate prognosis of patients with HPV16-positive HNSCCs. In this subgroup, viral load has stronger prognostic potential than P16 expression.pl
dc.affiliationWydział Lekarski : Zakład Wirusologiipl
dc.cm.date2020-01-07
dc.cm.id85997
dc.contributor.authorBiesaga, Beatapl
dc.contributor.authorMucha-Małecka, Annapl
dc.contributor.authorJanecka-Widła, Annapl
dc.contributor.authorKołodziej-Rzepa, Martapl
dc.contributor.authorSzostek, Sława - 133596 pl
dc.contributor.authorSłonina, Dorotapl
dc.contributor.authorKowalczyk, Aleksandrapl
dc.contributor.authorHalaszka, Krzysztofpl
dc.contributor.authorPrzewoźnik, Marcinpl
dc.date.accessioned2020-01-17T09:19:01Z
dc.date.available2020-01-17T09:19:01Z
dc.date.issued2018pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number1pl
dc.description.physical63-73pl
dc.description.points25pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume144pl
dc.identifier.doi10.1007/s00432-017-2531-2pl
dc.identifier.eissn1432-1335pl
dc.identifier.issn0171-5216pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/141740
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeinne
dc.subject.enHNSCCspl
dc.subject.enHPV16pl
dc.subject.enviral loadpl
dc.subject.enP16 expressionpl
dc.subject.enPrognosispl
dc.subtypeArticlepl
dc.titleDifferences in the prognosis of HPV16-positive patients with squamous cell carcinoma of head and neck according to viral load and expression of P16pl
dc.title.journalJournal of Cancer Research and Clinical Oncologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T20:30:32Z
dc.abstract.enpl
Purpose To evaluate the impact of HPV16 load (VL-the number of virus genome copies per cell) and P16 expression on prognosis of patients with squamous cell carcinomas (SCCs) of head and neck (HN). Materials and methods HPV16 presence was assessed in the group of 109 patients with HNSCCs by quantitative polymerase chain reaction (qPCR). VL (assessed by qPCR) and P16 expression (evaluated by immunohistochemistry) were analysed only in the subgroup of HPV16-positive tumours. These features were correlated with 5-year overall survival (OS) and disease-free survival (DFS). Results HPV16 infection was found in 36 tumours (33.0%). Virus-positive patients had better OS and DFS than those without infection (P = 0.041 and 0.005). Among HPV16-positive HNSCCs, 18 (50.0%) had higher VL (median value > 6764.3 copies/cell) and 25 (73.5%) P16 over expression. The significant differences in OS and DFS (P = 0.008 and 0.004) were noticed according to VL, wherein 100% DFS was found for patients with higher VL. According to P16 expression, significant difference was found only for OS (P = 0.020). In multivariate analysis, VL (P = 0.045; HR = 2.795; CI 0.121-1.060) and the level of smoking (P = 0.023, HR = 2.253; CI 1.124-4.514) were independent factors affecting DFS of HPV16-positive patients. Conclusion On the basis of viral load, it is possible to differentiate prognosis of patients with HPV16-positive HNSCCs. In this subgroup, viral load has stronger prognostic potential than P16 expression.
dc.affiliationpl
Wydział Lekarski : Zakład Wirusologii
dc.cm.date
2020-01-07
dc.cm.id
85997
dc.contributor.authorpl
Biesaga, Beata
dc.contributor.authorpl
Mucha-Małecka, Anna
dc.contributor.authorpl
Janecka-Widła, Anna
dc.contributor.authorpl
Kołodziej-Rzepa, Marta
dc.contributor.authorpl
Szostek, Sława - 133596
dc.contributor.authorpl
Słonina, Dorota
dc.contributor.authorpl
Kowalczyk, Aleksandra
dc.contributor.authorpl
Halaszka, Krzysztof
dc.contributor.authorpl
Przewoźnik, Marcin
dc.date.accessioned
2020-01-17T09:19:01Z
dc.date.available
2020-01-17T09:19:01Z
dc.date.issuedpl
2018
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
1
dc.description.physicalpl
63-73
dc.description.pointspl
25
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
144
dc.identifier.doipl
10.1007/s00432-017-2531-2
dc.identifier.eissnpl
1432-1335
dc.identifier.issnpl
0171-5216
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/141740
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
inne
dc.subject.enpl
HNSCCs
dc.subject.enpl
HPV16
dc.subject.enpl
viral load
dc.subject.enpl
P16 expression
dc.subject.enpl
Prognosis
dc.subtypepl
Article
dc.titlepl
Differences in the prognosis of HPV16-positive patients with squamous cell carcinoma of head and neck according to viral load and expression of P16
dc.title.journalpl
Journal of Cancer Research and Clinical Oncology
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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