Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy

2016
journal article
article
dc.abstract.enBackground Fibrosis of extracellular matrix (ECM) in dilated cardiomyopathy (DCM) corresponds to the myocardial over-production of various types of collagens. However, mechanism of this process is poorly understood. Objective To investigate whether enhanced metabolism of ECM occur in DCM. Methods Seventy consecutive DCM patients (pts) (48 ± 12.1 years, EF 24.4 ± 7.4 %) and 20 healthy volunteers were studied. Based on symptoms duration, pts were divided into new-onset (n = 35, 6 months) and chronic DCM (n = 35, >6 months). Markers of collagen type I and III synthesis-procollagen type I carboxy- and amino-terminal peptides (PICP and PINP) and procollagen type III carboxy- and amino-terminal peptides (PIIICP and PIIINP), collagen 1 (col-1), ECM metabolism controlling factors-tumor growth factor beta-1 (TGF1-b), connective tissue growth factor (CTGF), and ECM degradation enzymes-matrix metalloproteinases (MMP-2, MMP-9) and their tissue inhibitor (TIMP-1) were measured in serum. All pts underwent right ventricular endomyocardial biopsy to study ECM fibrosis. Results The presence of fibrosis was detected in 24 (34.3 %) pts and was more prevalent in chronic DCM [17 (48.6 %) vs. 7 (20 %), p\0.01]. The levels of PIIINP [4.41 (2.17-6.08) vs. 3.32 (1.69-5.02) ng/ml, p\0.001], CTGF [3.82 (0.48-23.87) vs. 2.37 (0.51-25.32) ng/ml, p\0.01], MMP-2 [6.06 (2.72-14.8) vs. 4.43 (2.27=7.4) ng/ml, p\0.001], MMP-9 [1.98 (0.28-9.25) vs. 1.01 (0.29-3.59) ng/ml, p\0.002)], and TIMP-1 [15.29 (1.8-36.17) vs. 2.61 (1.65-24.09) ng/ml, p\0.004] were significantly higher in DCM, whereas levels of col-1 [57.7 (23.1-233.4) vs. 159.4 (31.2-512.9) pg/ml, p\0.001] were significantly lower in DCM compared to controls. There were no differences in all measured serum markers of ECM metabolism between newonset and chronic DCM and as well as fibrosis positive and negative pts. Fibrosis was weakly correlated only with the duration of DCM (r = 0.23, p\0.05), however, not a single serum marker of fibrosis correlated with fibrosis. Neither unadjusted nor adjusted models, constructed from serum markers of ECM metabolism, predicted the probability of myocardial fibrosis. Conclusions Dynamics of ECM turnover in DCM is high, which is reflected by the increased levels CTGF and degradation enzymes. Synthesis of collagen type III prevailed over collagen type I. ECM metabolism was not different in DCM regardless of the duration of the disease and status of myocardial fibrosis. Serum markers of ECM metabolism were found not to be useful for the prediction of myocardial fibrosis in DCM.pl
dc.affiliationWydział Lekarski : Instytut Kardiologiipl
dc.cm.date2020-01-07
dc.cm.id79064
dc.contributor.authorRubiś, Paweł - 320209 pl
dc.contributor.authorWiśniowska-Śmiałek, Sylwia - 358423 pl
dc.contributor.authorWypasek, Ewa - 132801 pl
dc.contributor.authorBiernacka-Fijałkowska, Barbarapl
dc.contributor.authorRudnicka-Sosin, Lucynapl
dc.contributor.authorDziewięcka, Ewapl
dc.contributor.authorMatusik, Patrycja S.pl
dc.contributor.authorKhachatryana, Lusinepl
dc.contributor.authorKarabinowska, Aleksandrapl
dc.contributor.authorKozanecki, Arturpl
dc.contributor.authorTomkiewicz-Pająk, Lidia - 255811 pl
dc.contributor.authorPodolec, Piotr - 133185 pl
dc.date.accessioned2020-01-17T09:10:23Z
dc.date.available2020-01-17T09:10:23Z
dc.date.issued2016pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number12pl
dc.description.physical941-949pl
dc.description.points20pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume65pl
dc.identifier.doi10.1007/s00011-016-0977-3pl
dc.identifier.eissn1420-908Xpl
dc.identifier.issn1023-3830pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/138683
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeinne
dc.subject.endilated cardiomyopathypl
dc.subject.enextracellular matrixpl
dc.subject.enfibrosispl
dc.subject.enmarkerspl
dc.subject.encollagenpl
dc.subject.enbiopsypl
dc.subtypeArticlepl
dc.titleFibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathypl
dc.title.journalInflammation Researchpl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Background Fibrosis of extracellular matrix (ECM) in dilated cardiomyopathy (DCM) corresponds to the myocardial over-production of various types of collagens. However, mechanism of this process is poorly understood. Objective To investigate whether enhanced metabolism of ECM occur in DCM. Methods Seventy consecutive DCM patients (pts) (48 ± 12.1 years, EF 24.4 ± 7.4 %) and 20 healthy volunteers were studied. Based on symptoms duration, pts were divided into new-onset (n = 35, 6 months) and chronic DCM (n = 35, >6 months). Markers of collagen type I and III synthesis-procollagen type I carboxy- and amino-terminal peptides (PICP and PINP) and procollagen type III carboxy- and amino-terminal peptides (PIIICP and PIIINP), collagen 1 (col-1), ECM metabolism controlling factors-tumor growth factor beta-1 (TGF1-b), connective tissue growth factor (CTGF), and ECM degradation enzymes-matrix metalloproteinases (MMP-2, MMP-9) and their tissue inhibitor (TIMP-1) were measured in serum. All pts underwent right ventricular endomyocardial biopsy to study ECM fibrosis. Results The presence of fibrosis was detected in 24 (34.3 %) pts and was more prevalent in chronic DCM [17 (48.6 %) vs. 7 (20 %), p\0.01]. The levels of PIIINP [4.41 (2.17-6.08) vs. 3.32 (1.69-5.02) ng/ml, p\0.001], CTGF [3.82 (0.48-23.87) vs. 2.37 (0.51-25.32) ng/ml, p\0.01], MMP-2 [6.06 (2.72-14.8) vs. 4.43 (2.27=7.4) ng/ml, p\0.001], MMP-9 [1.98 (0.28-9.25) vs. 1.01 (0.29-3.59) ng/ml, p\0.002)], and TIMP-1 [15.29 (1.8-36.17) vs. 2.61 (1.65-24.09) ng/ml, p\0.004] were significantly higher in DCM, whereas levels of col-1 [57.7 (23.1-233.4) vs. 159.4 (31.2-512.9) pg/ml, p\0.001] were significantly lower in DCM compared to controls. There were no differences in all measured serum markers of ECM metabolism between newonset and chronic DCM and as well as fibrosis positive and negative pts. Fibrosis was weakly correlated only with the duration of DCM (r = 0.23, p\0.05), however, not a single serum marker of fibrosis correlated with fibrosis. Neither unadjusted nor adjusted models, constructed from serum markers of ECM metabolism, predicted the probability of myocardial fibrosis. Conclusions Dynamics of ECM turnover in DCM is high, which is reflected by the increased levels CTGF and degradation enzymes. Synthesis of collagen type III prevailed over collagen type I. ECM metabolism was not different in DCM regardless of the duration of the disease and status of myocardial fibrosis. Serum markers of ECM metabolism were found not to be useful for the prediction of myocardial fibrosis in DCM.
dc.affiliationpl
Wydział Lekarski : Instytut Kardiologii
dc.cm.date
2020-01-07
dc.cm.id
79064
dc.contributor.authorpl
Rubiś, Paweł - 320209
dc.contributor.authorpl
Wiśniowska-Śmiałek, Sylwia - 358423
dc.contributor.authorpl
Wypasek, Ewa - 132801
dc.contributor.authorpl
Biernacka-Fijałkowska, Barbara
dc.contributor.authorpl
Rudnicka-Sosin, Lucyna
dc.contributor.authorpl
Dziewięcka, Ewa
dc.contributor.authorpl
Matusik, Patrycja S.
dc.contributor.authorpl
Khachatryana, Lusine
dc.contributor.authorpl
Karabinowska, Aleksandra
dc.contributor.authorpl
Kozanecki, Artur
dc.contributor.authorpl
Tomkiewicz-Pająk, Lidia - 255811
dc.contributor.authorpl
Podolec, Piotr - 133185
dc.date.accessioned
2020-01-17T09:10:23Z
dc.date.available
2020-01-17T09:10:23Z
dc.date.issuedpl
2016
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
12
dc.description.physicalpl
941-949
dc.description.pointspl
20
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
65
dc.identifier.doipl
10.1007/s00011-016-0977-3
dc.identifier.eissnpl
1420-908X
dc.identifier.issnpl
1023-3830
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/138683
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
inne
dc.subject.enpl
dilated cardiomyopathy
dc.subject.enpl
extracellular matrix
dc.subject.enpl
fibrosis
dc.subject.enpl
markers
dc.subject.enpl
collagen
dc.subject.enpl
biopsy
dc.subtypepl
Article
dc.titlepl
Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy
dc.title.journalpl
Inflammation Research
dc.typepl
JournalArticle
dspace.entity.type
Publication

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