Multifaceted interplay between lipophilicity, protein interaction and luminescence parameters of non-intercalative ruthenium(II) polypyridyl complexes controlling cellular imaging and cytotoxic properties

2014
journal article
article
cris.lastimport.wos2024-04-10T02:05:00Z
dc.abstract.enHere, we examine the photophysical properties of five ruthenium(II) complexes comprising two 4,7-diphenyl-1,10-phenanthroline (dip) ligands and functionalized bipyridine (R1bpy-R2, where R1 = H or CH3, R2 = H, CH3, COO−,4-[3-(2-nitro-1H-imidazol-1-yl)propyl] or 1,3-dicyclohexyl-1-carbonyl-urea) towards development of luminescence probes for cellular imaging. These complexes have been shown to interact with albumin and the formed adducts exhibited up to eightfold increase in the luminescence quantum yield as well as the average lifetime of emission. It was demonstrated that they cannot bind to DNA through the intercalation mode and its luminescence in the presence of DNA is quenching. Cell viability experiments indicated that all complexes possess significant dose-dependent cytotoxicity (with IC50 5–19 μM) on 4T1 breast cancer cell line and their anti-proliferative activity correlates very well with their lipophilicity. Cellular uptake was studied by measuring the ruthenium content in cells using ICP-MS technique. As expected, the better uptake is directly related to higher lipophilicity of doubly charged ruthenium complexes while uptake of monocationic one is much lower in spite of the highest lipophilicity. Additionally staining properties were assessed using flow cytometry and fluorescence microscopy. These experiments showed that complex with 1,3-dicyclohexyl-1-carbonyl-urea substituent exhibits the best staining properties in spite of the lowest luminescence quantum yield in buffered solution (pH 7.4). Our results point out that both the imaging and cytotoxic properties of the studied ruthenium complexes are strongly influence by the level of internalization and protein interaction.pl
dc.affiliationWydział Chemii : Zakład Chemii Nieorganicznejpl
dc.affiliationWydział Chemii : Zakład Chemii Organicznejpl
dc.contributor.authorMazuryk, Olga - 124952 pl
dc.contributor.authorMagiera-Mularz, Katarzyna - 149547 pl
dc.contributor.authorRys, Barbara - 131753 pl
dc.contributor.authorSuzenet, Franckpl
dc.contributor.authorKieda, Claudinepl
dc.contributor.authorBrindell, Małgorzata - 127426 pl
dc.date.accession2018-12-03pl
dc.date.accessioned2015-06-23T06:15:44Z
dc.date.available2015-06-23T06:15:44Z
dc.date.issued2014pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalNa publikacji autorka podpisana: Magiera Katarzynapl
dc.description.admin[AB] Mazuryk, Olga 50000141pl
dc.description.number8pl
dc.description.physical1305-1316pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume19pl
dc.identifier.doi10.1007/s00775-014-1187-5pl
dc.identifier.eissn1432-1327pl
dc.identifier.issn0949-8257pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/10015
dc.identifier.weblinkhttps://link.springer.com/content/pdf/10.1007%2Fs00775-014-1187-5.pdfpl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeinne
dc.subtypeArticlepl
dc.titleMultifaceted interplay between lipophilicity, protein interaction and luminescence parameters of non-intercalative ruthenium(II) polypyridyl complexes controlling cellular imaging and cytotoxic propertiespl
dc.title.journalJBIC. Journal of Biological Inorganic Chemistrypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T02:05:00Z
dc.abstract.enpl
Here, we examine the photophysical properties of five ruthenium(II) complexes comprising two 4,7-diphenyl-1,10-phenanthroline (dip) ligands and functionalized bipyridine (R1bpy-R2, where R1 = H or CH3, R2 = H, CH3, COO−,4-[3-(2-nitro-1H-imidazol-1-yl)propyl] or 1,3-dicyclohexyl-1-carbonyl-urea) towards development of luminescence probes for cellular imaging. These complexes have been shown to interact with albumin and the formed adducts exhibited up to eightfold increase in the luminescence quantum yield as well as the average lifetime of emission. It was demonstrated that they cannot bind to DNA through the intercalation mode and its luminescence in the presence of DNA is quenching. Cell viability experiments indicated that all complexes possess significant dose-dependent cytotoxicity (with IC50 5–19 μM) on 4T1 breast cancer cell line and their anti-proliferative activity correlates very well with their lipophilicity. Cellular uptake was studied by measuring the ruthenium content in cells using ICP-MS technique. As expected, the better uptake is directly related to higher lipophilicity of doubly charged ruthenium complexes while uptake of monocationic one is much lower in spite of the highest lipophilicity. Additionally staining properties were assessed using flow cytometry and fluorescence microscopy. These experiments showed that complex with 1,3-dicyclohexyl-1-carbonyl-urea substituent exhibits the best staining properties in spite of the lowest luminescence quantum yield in buffered solution (pH 7.4). Our results point out that both the imaging and cytotoxic properties of the studied ruthenium complexes are strongly influence by the level of internalization and protein interaction.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Nieorganicznej
dc.affiliationpl
Wydział Chemii : Zakład Chemii Organicznej
dc.contributor.authorpl
Mazuryk, Olga - 124952
dc.contributor.authorpl
Magiera-Mularz, Katarzyna - 149547
dc.contributor.authorpl
Rys, Barbara - 131753
dc.contributor.authorpl
Suzenet, Franck
dc.contributor.authorpl
Kieda, Claudine
dc.contributor.authorpl
Brindell, Małgorzata - 127426
dc.date.accessionpl
2018-12-03
dc.date.accessioned
2015-06-23T06:15:44Z
dc.date.available
2015-06-23T06:15:44Z
dc.date.issuedpl
2014
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Na publikacji autorka podpisana: Magiera Katarzyna
dc.description.adminpl
[AB] Mazuryk, Olga 50000141
dc.description.numberpl
8
dc.description.physicalpl
1305-1316
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
19
dc.identifier.doipl
10.1007/s00775-014-1187-5
dc.identifier.eissnpl
1432-1327
dc.identifier.issnpl
0949-8257
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/10015
dc.identifier.weblinkpl
https://link.springer.com/content/pdf/10.1007%2Fs00775-014-1187-5.pdf
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
inne
dc.subtypepl
Article
dc.titlepl
Multifaceted interplay between lipophilicity, protein interaction and luminescence parameters of non-intercalative ruthenium(II) polypyridyl complexes controlling cellular imaging and cytotoxic properties
dc.title.journalpl
JBIC. Journal of Biological Inorganic Chemistry
dc.typepl
JournalArticle
dspace.entity.type
Publication

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