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Temperature-controlled orientation of proteins on temperature-responsive grafted polymer brushes : poly(butyl methacrylate) vs poly(butyl acrylate) : morphology, wetting, and protein adsorption
stimuli-responsive polymer coatings
grafted polymer brushes
glass transition temperature
orientation of proteins
PCA
ToF-SIMS
Poly(n-butyl methacrylate) (PBMA) or poly(n-butyl acrylate) (PBA)-grafted brush coatings attached to glass were successfully prepared using atom-transfer radical polymerization "from the surface". The thicknesses and composition of the PBMA and PBA coatings were examined using ellipsometry and time-of-flight secondary ion mass spectrometry (ToF-SIMS), respectively. For PBMA, the glass-transition temperature constitutes a range close to the physiological limit, which is in contrast to PBA, where the glass-transition temperature is around −55 °C. Atomic force microscopy studies at different temperatures suggest a strong morphological transformation for PBMA coatings, in contrast to PBA, where such essential changes in the surface morphology are absent. Besides, for PBMA coatings, protein adsorption depicts a strong temperature dependence. The combination of bovine serum albumin and anti-IgG structure analysis with the principal component analysis of ToF-SIMS spectra revealed a different orientation of proteins adsorbed to PBMA coatings at different temperatures. In addition, the biological activity of anti-IgG molecules adsorbed at different temperatures was evaluated through tracing the specific binding with goat IgG.
cris.lastimport.scopus | 2024-04-07T16:31:39Z | |
dc.abstract.en | Poly(n-butyl methacrylate) (PBMA) or poly(n-butyl acrylate) (PBA)-grafted brush coatings attached to glass were successfully prepared using atom-transfer radical polymerization "from the surface". The thicknesses and composition of the PBMA and PBA coatings were examined using ellipsometry and time-of-flight secondary ion mass spectrometry (ToF-SIMS), respectively. For PBMA, the glass-transition temperature constitutes a range close to the physiological limit, which is in contrast to PBA, where the glass-transition temperature is around −55 °C. Atomic force microscopy studies at different temperatures suggest a strong morphological transformation for PBMA coatings, in contrast to PBA, where such essential changes in the surface morphology are absent. Besides, for PBMA coatings, protein adsorption depicts a strong temperature dependence. The combination of bovine serum albumin and anti-IgG structure analysis with the principal component analysis of ToF-SIMS spectra revealed a different orientation of proteins adsorbed to PBMA coatings at different temperatures. In addition, the biological activity of anti-IgG molecules adsorbed at different temperatures was evaluated through tracing the specific binding with goat IgG. | pl |
dc.affiliation | Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego | pl |
dc.contributor.author | Awsiuk, Kamil - 103592 | pl |
dc.contributor.author | Yurij, Stetsyshyn | pl |
dc.contributor.author | Raczkowska, Joanna - 102150 | pl |
dc.contributor.author | Ostap, Lishchynskyi | pl |
dc.contributor.author | Dąbczyński, Paweł - 190151 | pl |
dc.contributor.author | Andrij, Kostruba | pl |
dc.contributor.author | Halyna, Ohar | pl |
dc.contributor.author | Yana, Shymborska | pl |
dc.contributor.author | Nastyshyn, Svyatoslav - 408127 | pl |
dc.contributor.author | Budkowski, Andrzej - 127461 | pl |
dc.date.accessioned | 2019-09-04T06:43:21Z | |
dc.date.available | 2019-09-04T06:43:21Z | |
dc.date.issued | 2019 | pl |
dc.description.number | 6 | pl |
dc.description.physical | 2185-2197 | pl |
dc.description.volume | 20 | pl |
dc.identifier.doi | 10.1021/acs.biomac.9b00030 | pl |
dc.identifier.eissn | 1526-4602 | pl |
dc.identifier.issn | 1525-7797 | pl |
dc.identifier.project | ROD UJ / O | pl |
dc.identifier.uri | https://ruj.uj.edu.pl/xmlui/handle/item/81738 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Dodaję tylko opis bibliograficzny | * |
dc.rights.licence | bez licencji | |
dc.rights.uri | * | |
dc.subject.en | stimuli-responsive polymer coatings | pl |
dc.subject.en | grafted polymer brushes | pl |
dc.subject.en | glass transition temperature | pl |
dc.subject.en | orientation of proteins | pl |
dc.subject.en | PCA | pl |
dc.subject.en | ToF-SIMS | pl |
dc.subtype | Article | pl |
dc.title | Temperature-controlled orientation of proteins on temperature-responsive grafted polymer brushes : poly(butyl methacrylate) vs poly(butyl acrylate) : morphology, wetting, and protein adsorption | pl |
dc.title.journal | Biomacromolecules | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |