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The lectin-binding pattern of nucleolin and its interaction with endogenous galectin-3
glycosylation of nucleolin
galectin-3
melanoma
mass spectrometry
confocal microscopy
lectin assay
co-immunoprecipitation
Unlike nuclear nucleolin, surface-expressed and cytoplasmic nucleolin exhibit Tn antigen. Here, we show localization-dependent differences in the glycosylation and proteolysis patterns of nucleolin. Our results provide evidence for different paths of nucleolin proteolysis in the nucleus, in the cytoplasm, and on the cell surface. We found that full-length nucleolin and some proteolytic fragments coexist within live cells and are not solely the result of the preparation procedure. Extranuclear nucleolin undergoes N- and O-glycosylation, and unlike cytoplasmic nucleolin, membrane-associated nucleolin is not fucosylated. Here, we show for the first time that nucleolin and endogenous galectin-3 exist in the same complexes in the nucleolus, the cytoplasm, and on the cell surface of melanoma cells. Assessments of the interaction of nucleolin with galectin-3 revealed nucleolar co-localization in interphase, suggesting that galectin-3 may be involved in DNA organization and ribosome biogenesis.
cris.lastimport.wos | 2024-04-09T20:32:03Z | |
dc.abstract.en | Unlike nuclear nucleolin, surface-expressed and cytoplasmic nucleolin exhibit Tn antigen. Here, we show localization-dependent differences in the glycosylation and proteolysis patterns of nucleolin. Our results provide evidence for different paths of nucleolin proteolysis in the nucleus, in the cytoplasm, and on the cell surface. We found that full-length nucleolin and some proteolytic fragments coexist within live cells and are not solely the result of the preparation procedure. Extranuclear nucleolin undergoes N- and O-glycosylation, and unlike cytoplasmic nucleolin, membrane-associated nucleolin is not fucosylated. Here, we show for the first time that nucleolin and endogenous galectin-3 exist in the same complexes in the nucleolus, the cytoplasm, and on the cell surface of melanoma cells. Assessments of the interaction of nucleolin with galectin-3 revealed nucleolar co-localization in interphase, suggesting that galectin-3 may be involved in DNA organization and ribosome biogenesis. | pl |
dc.affiliation | Wydział Biologii i Nauk o Ziemi : Instytut Zoologii | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej | pl |
dc.contributor.author | Hoja-Łukowicz, Dorota - 128335 | pl |
dc.contributor.author | Kędracka-Krok, Sylwia - 128739 | pl |
dc.contributor.author | Duda, Weronika | pl |
dc.contributor.author | Lityńska, Anna - 130002 | pl |
dc.date.accessioned | 2015-04-20T13:12:33Z | |
dc.date.available | 2015-04-20T13:12:33Z | |
dc.date.issued | 2014 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.additional | Bibliogr. s. 478-482 | pl |
dc.description.number | 3 | pl |
dc.description.physical | 461-482 | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 19 | pl |
dc.identifier.doi | 10.2478/s11658-014-0206-4 | pl |
dc.identifier.eissn | 1689-1392 | pl |
dc.identifier.issn | 1425-8153 | pl |
dc.identifier.project | ROD UJ / P | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/5309 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska | * |
dc.rights.licence | CC-BY-NC-ND | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/pl/legalcode | * |
dc.share.type | inne | |
dc.subject.en | glycosylation of nucleolin | pl |
dc.subject.en | galectin-3 | pl |
dc.subject.en | melanoma | pl |
dc.subject.en | mass spectrometry | pl |
dc.subject.en | confocal microscopy | pl |
dc.subject.en | lectin assay | pl |
dc.subject.en | co-immunoprecipitation | pl |
dc.subtype | Article | pl |
dc.title | The lectin-binding pattern of nucleolin and its interaction with endogenous galectin-3 | pl |
dc.title.journal | Cellular & Molecular Biology Letters | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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