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A capillary electrophoretic (CE) method for screening of six tricyclic antidepressants: amitriptyline,
nortriptyline, imipramine, desipramine, doxepin and nordoxepin, in human serum, has been developed. The
drugs were separated in a bare fused silica capillary (50 μm i.d.) using the background electrolyte: 50 mM
CAPSO (pH 9.54) in methanol/water with KCl addition. For increasing the method sensitivity, the sample con-
centration in the capillary (sample stacking) using pressure and electrokinetic injection has been applied. The
standard addition method was used for calibration of the developed analytical procedure. The precision of the
identification parameter (the relative migration time) and the quantitative parameter (the relative peak area) was
within the range of 0.05ñ1.65 and 0.73ñ6.7 (RSD %), respectively. The detection limits were found to be 30
ng/mL for desipramine, 62.5 ng/mL for nortriptyline, and 50 ng/mL for remaining analytes.
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dc.subject.en
capillary electrophoresis
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dc.subject.en
standard addition calibration
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dc.subject.en
ricyclic antidepressants
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dc.subject.en
human serum
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dc.subject.en
stacking
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dc.description.volume
69
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dc.description.number
6
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dc.description.points
15
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dc.identifier.eissn
2353-5288
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dc.title.journal
Acta Poloniae Pharmaceutica. Drug Research
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dc.language.container
eng
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dc.date.accession
2016-05-27
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dc.affiliation
Wydział Chemii : Zakład Chemii Analitycznej
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dc.subtype
Article
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dc.rights.original
CC-BY-NC; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0