Simple view
Full metadata view
Authors
Statistics
Photodegradation assessment of ciprofloxacin, moxifloxacin, norfloxacin and ofloxacin in the presence of excipients from tablets by UPLC-MS/MS and DSC
ciprofloxacin
moxifloxacin
norfloxacin
ofloxacin
photodegradation
kinetic evaluation
ultra performance liquid chromatography
tandem mass spectrometry
differential scanning calorimetry
Background: Ciprofloxacin (CIP), moxifloxacin (MOX), norfloxacin (NOR) and ofloxacin (OFL), are the antibacterial synthetic drugs, belonging to the fluoroquinolones group. Fluoroquinolones are compounds susceptible to photodegradation process, which may lead to reduction of their antibacterial activity and to induce phototoxicity as a side effect. This paper describes a simple, sensitive UPLC-MS/MS method for the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products. Results: Chromatographic separations were carried out using the Acquity UPLC BEH C18 column; (2.1 × 100 mm, 1.7 μm particle size). The column was maintained at 40°C, and the following gradient was used: 0 min, 95% of eluent A and 5% of eluent B; 10 min, 0% of eluent A and 100% of eluent B, at a flow rate of 0.3 mL min-1. Eluent A: 0.1% (v/v) formic acid in water; eluent B: 0.1% (v/v) formic acid in acetonitrile. The method was validated and all the validation parameters were in the ranges acceptable by the guidelines for analytical method validation. The photodegradation of examined fluoroquinolones in solid phase in the presence of excipients followed kinetic of the first order reaction and depended upon the type of analyzed drugs and coexisting substances. Photodegradation process of analyzed drugs was confirmed by differential scanning calorimetry. In addition, the identification of degradation products was carried out by mass spectrometry. Conclusion: The developed UPLC-MS/MS method enables the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products and identification of photodegradation products.
dc.abstract.en | Background: Ciprofloxacin (CIP), moxifloxacin (MOX), norfloxacin (NOR) and ofloxacin (OFL), are the antibacterial synthetic drugs, belonging to the fluoroquinolones group. Fluoroquinolones are compounds susceptible to photodegradation process, which may lead to reduction of their antibacterial activity and to induce phototoxicity as a side effect. This paper describes a simple, sensitive UPLC-MS/MS method for the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products. Results: Chromatographic separations were carried out using the Acquity UPLC BEH C18 column; (2.1 × 100 mm, 1.7 μm particle size). The column was maintained at 40°C, and the following gradient was used: 0 min, 95% of eluent A and 5% of eluent B; 10 min, 0% of eluent A and 100% of eluent B, at a flow rate of 0.3 mL min-1. Eluent A: 0.1% (v/v) formic acid in water; eluent B: 0.1% (v/v) formic acid in acetonitrile. The method was validated and all the validation parameters were in the ranges acceptable by the guidelines for analytical method validation. The photodegradation of examined fluoroquinolones in solid phase in the presence of excipients followed kinetic of the first order reaction and depended upon the type of analyzed drugs and coexisting substances. Photodegradation process of analyzed drugs was confirmed by differential scanning calorimetry. In addition, the identification of degradation products was carried out by mass spectrometry. Conclusion: The developed UPLC-MS/MS method enables the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products and identification of photodegradation products. | pl |
dc.affiliation | Wydział Farmaceutyczny : Zakład Chemii Analitycznej | pl |
dc.affiliation | Wydział Farmaceutyczny : Zakład Chemii Leków | pl |
dc.cm.date | 2020-01-07 | |
dc.cm.id | 59900 | |
dc.contributor.author | Hubicka, Urszula - 129734 | pl |
dc.contributor.author | Żmudzki, Paweł - 214411 | pl |
dc.contributor.author | Talik, Przemysław - 200522 | pl |
dc.contributor.author | Żuromska-Witek, Barbara - 200539 | pl |
dc.contributor.author | Krzek, Jan - 130468 | pl |
dc.date.accessioned | 2020-01-17T07:52:09Z | |
dc.date.available | 2020-01-17T07:52:09Z | |
dc.date.issued | 2013 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.points | 25 | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 7 | pl |
dc.identifier.articleid | 133 | pl |
dc.identifier.doi | 10.1186/1752-153X-7-133 | pl |
dc.identifier.eissn | 1752-153X | |
dc.identifier.project | ROD UJ / OP | pl |
dc.identifier.uri | https://ruj.uj.edu.pl/xmlui/handle/item/131871 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa 2.0 | * |
dc.rights.licence | CC-BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0/pl/legalcode | * |
dc.share.type | otwarte czasopismo | |
dc.subject.en | ciprofloxacin | pl |
dc.subject.en | moxifloxacin | pl |
dc.subject.en | norfloxacin | pl |
dc.subject.en | ofloxacin | pl |
dc.subject.en | photodegradation | pl |
dc.subject.en | kinetic evaluation | pl |
dc.subject.en | ultra performance liquid chromatography | pl |
dc.subject.en | tandem mass spectrometry | pl |
dc.subject.en | differential scanning calorimetry | pl |
dc.subtype | Article | pl |
dc.title | Photodegradation assessment of ciprofloxacin, moxifloxacin, norfloxacin and ofloxacin in the presence of excipients from tablets by UPLC-MS/MS and DSC | pl |
dc.title.journal | Chemistry Central Journal | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.
Views
14
Views per month
Views per city
Downloads
Open Access