tytuł:
|
Antigen-specific, antibody-coated, exosome-like nanovesicles deliver suppressor T-cell microRNA-150 to effector T cells to inhibit contact sensitivity |
autor: |
Bryniarski Krzysztof , Ptak Włodzimierz, Jayakumar Asha, Pullmann Kerstin, Caplan Michael J., Chairoungdua Arthit, Lu Jun, Adams Brian D., Martin Emilia , Nazimek Katarzyna , Marquez Susanna, Kleinstein Steven H., Sangwung Panjamaporn, Iwakiri Yasuko, Delgato Eric, Redegeld Frank, Blokhuis Bart R., Wojcikowski Jacek, Daniel Władysława Anna, Groot Kormelink Tom, Askenase Philip William |
tytuł czasopisma:
|
Journal of Allergy and Clinical Immunology |
tom: |
132 |
numer:
|
1 |
data wydania
:
|
2013 |
strony:
|
170-181 |
ISSN: |
0091-6749
|
eISSN: |
1097-6825
|
DOI: |
10.1016/j.jaci.2013.04.048
|
uwagi:
|
Autorka Emilia Martin podpisana: Sikora, Emilia |
język: |
angielski |
język czasopisma:
|
angielski |
abstrakt w j. angielskim: |
Background: T-cell tolerance of allergic cutaneous contact
sensitivity (CS) induced in mice by high doses of reactive hapten
is mediated by suppressor cells that release antigen-specific
suppressive nanovesicles.
Objective: We sought to determine the mechanism or
mechanisms of immune suppression mediated by the
nanovesicles.
Methods: T-cell tolerance was induced by means of intravenous
injection of hapten conjugated to self-antigens of syngeneic
erythrocytes and subsequent contact immunization with the same
hapten. Lymph node and spleen cells from tolerized or control
donors were harvested and cultured to produce a supernatant
containing suppressive nanovesicles that were isolated from the
tolerized mice for testing in active and adoptive cell-transfer
models of CS.
Results: Tolerance was shown due to exosome-like nanovesicles
in the supernatants of CD81 suppressor T cells that were not
regulatory T cells. Antigen specificity of the suppressive
nanovesicles was conferred by a surface coat of antibody light
chains or possibly whole antibody, allowing targeted delivery of
selected inhibitory microRNA (miRNA)–150 to CS effector T
cells. Nanovesicles also inhibited CS in actively sensitized mice
after systemic injection at the peak of the responses. The role of
antibody and miRNA-150 was established by tolerizing either
panimmunoglobulin-deficient JH2/2 or miRNA-1502/2 mice
that produced nonsuppressive nanovesicles. These nanovesicles
could be made suppressive by adding antigen-specific antibody
light chains or miRNA-150, respectively.
Conclusions: This is the first example of T-cell regulation
through systemic transit of exosome-like nanovesicles delivering
a chosen inhibitory miRNA to target effector T cells in an
antigen-specific manner by a surface coating of antibody light
chains. |
słowa kluczowe w j. angielskim: |
exosomes, exosome-like nanovesicles, nanovesicles, T-cell suppression, miRNA, miRNA-150, antibody light chains, allergic cutaneous contact dermatitis, contact sensitivity |
punktacja wydziałowa: |
50 |
wydział: instytut / zakład / katedra: |
Wydział Lekarski : Katedra Immunologii, Wydział Lekarski : Zakład Immunologii |
typ: |
artykuł w czasopiśmie |
podtyp: |
artykuł |
punktacja MEiN [2013 A]: 50