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Lactoferrin is considered as a part of the innate
immune system that plays a crucial role in preventing bac-
terial growth, mostly via an iron sequestration mechanism.
Recent data show that bovine lactoferrin prevents late-onset
sepsis in preterm very low birth weight neonates by serving
as an iron chelator for some bacterial strains; thus, it is very
important to control the iron saturation level during diet
supplementation. An accurate estimation of lactoferrin iron
saturation is essential not only because of its clinical appli-
cations but also for a wide range of biochemical experi-
ments. A comprehensive method for the quantification of
iron saturation in lactoferrin preparations was developed to
obtain a calibration curve enabling the determination of iron
saturation levels relying exclusively on the defined ratio of
absorbances at 280 and 466 nm (
A
280/466
). To achieve this
goal, selected techniques such as spectrophotometry, ELISA,and ICP-MS were combined. The ability to obtain samples of
lactoferrin with determination of its iron content in a simple
and fast way has beenproven tobe veryuseful. Furthermore, a
similar approach could easily be implemented to facilitate the
determination of iron saturation level for other metalloproteins
in which metal binding results in the appearance of a distinct
band in the visible part of the spectrum.
słowa kluczowe w j. angielskim:
lactoferrin, iron saturation, absorption ratio, ICP-MS
punktacja wydziałowa:
40
wydział: instytut / zakład / katedra:
Wydział Chemii : Zakład Chemii Nieorganicznej, Wydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologii, Wydział Chemii : Zakład Krystalochemii i Krystalofizyki