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UV-induced spectral shift and protonation of DNA fluorescent dye Hoechst 33258
photoconversion
Hoechst
DNA
super-resolution microscopy
DNA-bound Hoechst 33258 is readily excited with UV light and emits blue fluorescence, however, upon exposure to UV, the dye undergoes photobleaching as well as photoconversion to a blue-excited green-emitting form. We demonstrate that the UV-generated green-emitting form of Hoechst 33258 exhibits spectral properties very similar to the form of the dye that can be obtained by subjecting it to an acidic environment (pH 0.5–3.0). We also demonstrate that exposure of Hoechst 33258 to UV light (or hydrogen peroxide) leads to generation of the protonated (1+, 2+, 3+ and possibly the 4+) forms of the dye. Photoconversion of Hoechst 33258 has recently been exploited in single molecule localisation microscopy, thus understanding photophysics of this process can facilitate further development of high resolution optical imaging.
cris.lastimport.wos | 2024-04-10T01:08:19Z | |
dc.abstract.en | DNA-bound Hoechst 33258 is readily excited with UV light and emits blue fluorescence, however, upon exposure to UV, the dye undergoes photobleaching as well as photoconversion to a blue-excited green-emitting form. We demonstrate that the UV-generated green-emitting form of Hoechst 33258 exhibits spectral properties very similar to the form of the dye that can be obtained by subjecting it to an acidic environment (pH 0.5–3.0). We also demonstrate that exposure of Hoechst 33258 to UV light (or hydrogen peroxide) leads to generation of the protonated (1+, 2+, 3+ and possibly the 4+) forms of the dye. Photoconversion of Hoechst 33258 has recently been exploited in single molecule localisation microscopy, thus understanding photophysics of this process can facilitate further development of high resolution optical imaging. | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Pracownia Biofizyki Komórki | pl |
dc.contributor.author | Żurek-Biesiada, Dominika - 108768 | pl |
dc.contributor.author | Waligórski, Piotr | pl |
dc.contributor.author | Dobrucki, Jerzy - 127740 | pl |
dc.date.accession | 2019-02-14 | pl |
dc.date.accessioned | 2015-05-08T09:44:48Z | |
dc.date.available | 2015-05-08T09:44:48Z | |
dc.date.issued | 2014 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.additional | Bibliogr. s. 1801. Jerzy Dobrucki podpisany: Jurek W. Dobrucki | pl |
dc.description.number | 6 | pl |
dc.description.physical | 1791-1801 | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 24 | pl |
dc.identifier.doi | 10.1007/s10895-014-1468-y | pl |
dc.identifier.eissn | 1573-4994 | pl |
dc.identifier.issn | 1053-0509 | pl |
dc.identifier.project | ROD UJ / P | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/6754 | |
dc.identifier.weblink | https://link.springer.com/content/pdf/10.1007%2Fs10895-014-1468-y.pdf | pl |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa | * |
dc.rights.licence | CC-BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/legalcode.pl | * |
dc.share.type | inne | |
dc.subject.en | photoconversion | pl |
dc.subject.en | Hoechst | pl |
dc.subject.en | DNA | pl |
dc.subject.en | super-resolution microscopy | pl |
dc.subtype | Article | pl |
dc.title | UV-induced spectral shift and protonation of DNA fluorescent dye Hoechst 33258 | pl |
dc.title.journal | Journal of Fluorescence | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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Except as otherwise noted, this item is licensed under the Attribution 4.0 International licence