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Strain specific immunostimulatory potential of lactobacilli-derived exopolysaccharides
cytokines
macrophages
lipoteichoic acid (LTA)
exopolysaccharide (EPS)
probiotics
lactobacilli
Lactobacilli, the most commonly used probiotics, show strain specific immunostimulatory effects. The strain specificity is related to the structure of cell-wall components such as lipoteichoic acids (LTAs), ligands for TLR2. In contrast, the role of exopolysaccharides (EPSs), the major components of lactobacilli biofilm, in the “cross-talk” between bacteria and the immune system is poorly documented. In this study, we have tested immunomodulating potential of lactobacilli derived EPSs, by their ability to modulate in vitro production of cytokines by murine peritoneal macrophages. We have examined EPSs of three lactobacilli strains (L. reuteri, L. johnsonii, L. animalis/murinus) isolated from colitic mice. The effect was compared with that of whole bacteria and LTA. All three nonviable Lactobacillus strains induced high amounts of cytokines with a strain specific profile. In contrast, the tested exopolysaccharides, exert low, albeit highly strain diversified stimulatory activities. The immunostimulatory potential of EPSs was correlated with the interaction strength between EPS and macrophage receptors as measured by atomic force spectroscopy.
dc.abstract.en | Lactobacilli, the most commonly used probiotics, show strain specific immunostimulatory effects. The strain specificity is related to the structure of cell-wall components such as lipoteichoic acids (LTAs), ligands for TLR2. In contrast, the role of exopolysaccharides (EPSs), the major components of lactobacilli biofilm, in the “cross-talk” between bacteria and the immune system is poorly documented. In this study, we have tested immunomodulating potential of lactobacilli derived EPSs, by their ability to modulate in vitro production of cytokines by murine peritoneal macrophages. We have examined EPSs of three lactobacilli strains (L. reuteri, L. johnsonii, L. animalis/murinus) isolated from colitic mice. The effect was compared with that of whole bacteria and LTA. All three nonviable Lactobacillus strains induced high amounts of cytokines with a strain specific profile. In contrast, the tested exopolysaccharides, exert low, albeit highly strain diversified stimulatory activities. The immunostimulatory potential of EPSs was correlated with the interaction strength between EPS and macrophage receptors as measured by atomic force spectroscopy. | pl |
dc.affiliation | Wydział Lekarski : Zakład Immunologii | pl |
dc.affiliation | Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego | pl |
dc.affiliation | Wydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologii | pl |
dc.contributor.author | Ciszek-Lenda, Marta - 129072 | pl |
dc.contributor.author | Strus, Magdalena - 133523 | pl |
dc.contributor.author | Górska-Frączek, Sabina | pl |
dc.contributor.author | Targosz-Korecka, Marta - 102459 | pl |
dc.contributor.author | Śróttek, Małgorzata - 128792 | pl |
dc.contributor.author | Heczko, Piotr - 129700 | pl |
dc.contributor.author | Gamian, Andrzej | pl |
dc.contributor.author | Szymoński, Marek - 132296 | pl |
dc.contributor.author | Marcinkiewicz, Janusz - 130850 | pl |
dc.date.accession | 2017-01-03 | pl |
dc.date.accessioned | 2017-01-03T13:51:06Z | |
dc.date.available | 2017-01-03T13:51:06Z | |
dc.date.issued | 2011 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.number | 3 | pl |
dc.description.physical | 121-129 | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 36 | pl |
dc.identifier.eissn | 1644-4124 | pl |
dc.identifier.issn | 1426-3912 | pl |
dc.identifier.project | ROD UJ / OP | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/34835 | |
dc.identifier.weblink | http://www.termedia.pl/-Experimental-immunology-Strain-specific-immunostimulatory-potential-of-lactobacilli-derived-exopolysaccharides,10,17497,1,1.html | pl |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa | * |
dc.rights.licence | CC-BY-NC-SA | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.pl | * |
dc.share.type | otwarte czasopismo | |
dc.subject.en | cytokines | pl |
dc.subject.en | macrophages | pl |
dc.subject.en | lipoteichoic acid (LTA) | pl |
dc.subject.en | exopolysaccharide (EPS) | pl |
dc.subject.en | probiotics | pl |
dc.subject.en | lactobacilli | pl |
dc.subtype | Article | pl |
dc.title | Strain specific immunostimulatory potential of lactobacilli-derived exopolysaccharides | pl |
dc.title.journal | Central-European Journal of Immunology | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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