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Endothelial glycocalyx integrity is preserved in young, healthy men during a single bout of strenuous physical exercise
endothelium
exercise
glycocalyx
inflammation
oxidative stress
In the present study we aimed to evaluate whether oxidative stress and inflammation induced by strenuous exercise affect glycocalyx integrity and endothelial function. Twenty one young, untrained healthy men performed a maximal incremental cycling exercise - until exhaustion. Markers of glycocalyx shedding (syndecan-1, heparan sulfate and hyaluronic acid), endothelial status (nitric oxide and prostacyclin metabolites - nitrate, nitrite, 6-keto-prostaglandin F1a), oxidative stress (8-oxo-2'- deoxyguanosine) and antioxidant capacity (uric acid, nonenzymatic antioxidant capacity) as well as markers of inflammation (sVCAM-1 and sICAM-1) were analyzed in venous blood samples taken at rest and at the end of exercise. The applied strenuous exercise caused a 5-fold increase in plasma lactate and hypoxanthine concentrations (p<0.001), a fall in plasma uric acid concentration and non-enzymatic antioxidant capacity (p<10-4), accompanied by an increase (p=0.003) in sVCAM-1 concentration. Plasma 6-keto-prostaglandin F1a concentration increased (p=0.006) at exhaustion, while nitrate and nitrite concentrations were not affected. Surprisingly, no significant changes in serum syndecan-1 and heparan sulfate concentrations were observed. We have concluded, that a single bout of severe-intensity exercise is well accommodated by endothelium in young, healthy men as it neither results in evident glycocalyx disruption nor in the impairment of nitric oxide and prostacyclin production.
dc.abstract.en | In the present study we aimed to evaluate whether oxidative stress and inflammation induced by strenuous exercise affect glycocalyx integrity and endothelial function. Twenty one young, untrained healthy men performed a maximal incremental cycling exercise - until exhaustion. Markers of glycocalyx shedding (syndecan-1, heparan sulfate and hyaluronic acid), endothelial status (nitric oxide and prostacyclin metabolites - nitrate, nitrite, 6-keto-prostaglandin F1a), oxidative stress (8-oxo-2'- deoxyguanosine) and antioxidant capacity (uric acid, nonenzymatic antioxidant capacity) as well as markers of inflammation (sVCAM-1 and sICAM-1) were analyzed in venous blood samples taken at rest and at the end of exercise. The applied strenuous exercise caused a 5-fold increase in plasma lactate and hypoxanthine concentrations (p<0.001), a fall in plasma uric acid concentration and non-enzymatic antioxidant capacity (p<10-4), accompanied by an increase (p=0.003) in sVCAM-1 concentration. Plasma 6-keto-prostaglandin F1a concentration increased (p=0.006) at exhaustion, while nitrate and nitrite concentrations were not affected. Surprisingly, no significant changes in serum syndecan-1 and heparan sulfate concentrations were observed. We have concluded, that a single bout of severe-intensity exercise is well accommodated by endothelium in young, healthy men as it neither results in evident glycocalyx disruption nor in the impairment of nitric oxide and prostacyclin production. | pl |
dc.affiliation | Pion Rektora : Jagiellońskie Centrum Rozwoju Leków | pl |
dc.affiliation | Wydział Lekarski : Zakład Farmakologii | pl |
dc.cm.id | 75180 | |
dc.contributor.author | Majerczak, J. | pl |
dc.contributor.author | Duda, K. | pl |
dc.contributor.author | Chłopicki, Stefan - 128995 | pl |
dc.contributor.author | Bartosz, G. | pl |
dc.contributor.author | Zakrzewska, Agnieszka - 198214 | pl |
dc.contributor.author | Balcerczyk, A. | pl |
dc.contributor.author | Smoleński, R. T. | pl |
dc.contributor.author | Zoladz, J. A. | pl |
dc.date.accession | 2016-08-24 | pl |
dc.date.accessioned | 2016-08-24T09:19:01Z | |
dc.date.available | 2016-08-24T09:19:01Z | |
dc.date.issued | 2016 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.number | 2 | pl |
dc.description.physical | 281-291 | pl |
dc.description.points | 20 | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 65 | pl |
dc.identifier.eissn | 1802-9973 | pl |
dc.identifier.issn | 0862-8408 | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/29819 | |
dc.identifier.weblink | http://www.biomed.cas.cz/physiolres/pdf/65/65_281.pdf | pl |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Dodaję tylko opis bibliograficzny | * |
dc.rights.licence | OTHER | |
dc.rights.uri | * | |
dc.share.type | otwarte czasopismo | |
dc.subject.en | endothelium | pl |
dc.subject.en | exercise | pl |
dc.subject.en | glycocalyx | pl |
dc.subject.en | inflammation | pl |
dc.subject.en | oxidative stress | pl |
dc.subtype | Article | pl |
dc.title | Endothelial glycocalyx integrity is preserved in young, healthy men during a single bout of strenuous physical exercise | pl |
dc.title.journal | Physiological Research | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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