tytuł:
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Effects of 1-methylnicotinamide (MNA) on exercise capacity and endothelial response in diabetic mice |
autor: |
Przyborowski Kamil , Wojewoda Marta , Sitek Barbara , Zakrzewska Agnieszka , Kij Agnieszka , Wandzel Krystyna , Zoladz Jerzy Andrzej, Chłopicki Stefan
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tytuł czasopisma:
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PLoS ONE |
tom: |
10 |
numer:
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6 |
data wydania
:
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2015 |
strony:
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[1-15] |
article ID:
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e0130908 |
eISSN: |
1932-6203
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DOI: |
10.1371/journal.pone.0130908
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język: |
angielski |
język czasopisma:
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angielski |
abstrakt w j. angielskim: |
1-Methylnicotinamide (MNA), which was initially considered to be a biologically inactive endogenous metabolite of nicotinamide, has emerged as an anti-thrombotic and anti-inflammatory agent with the capacity to release prostacyclin (PGI<inf>2</inf>). In the present study, we characterized the effects of MNA on exercise capacity and the endothelial response to exercise in diabetic mice. Eight-week-old db/db mice were untreated or treated with MNA for 4 weeks (100 mg·kg-1), and their exercise capacity as well as NO- and PGI<inf>2</inf>-dependent response to endurance running were subsequently assessed. MNA treatment of db/db mice resulted in four-fold and three-fold elevation of urine concentrations of MNA and its metabolites (Met-2PY + Met-4PY), respectively (P<0.01), but did not affect HbA<inf>1c</inf> concentration, fasting glucose concentration or lipid profile. However, insulin sensitivity was improved (P<0.01). In MNA-treated db/db mice, the time to fatigue for endurance exercise was significantly prolonged (P<0.05). Post-exercise Δ6-keto-PGF<inf>1α</inf> (difference between mean concentration in the sedentary and exercised groups) tended to increase, and post-exercise leukocytosis was substantially reduced in MNA-treated animals. In turn, the post-exercise fall in plasma concentration of nitrate was not affected by MNA. In conclusion, we demonstrated for the first time that MNA improves endurance exercise capacity in mice with diabetes, and may also decrease the cardiovascular risk of exercise. |
słowa kluczowe w j. angielskim: |
1-methylnicotinamide, 6-oxoprostaglandin F1 alpha, nitrate, nitrite, animal experiment, article, cardiovascular risk, controlled study, diabetes mellitus, drug blood level, endurance training, exercise intensity, heart protection, insulin sensitivity, leukocytosis, male, mouse, nonhuman, physical capacity, running, treatment response, vascular endothelium, animalia, mus |
punktacja wydziałowa: |
40 |
wydział: instytut / zakład / katedra: |
Pion Rektora : Jagiellońskie Centrum Rozwoju Leków, Wydział Lekarski : Zakład Farmakologii |
typ: |
artykuł w czasopiśmie |
podtyp: |
artykuł |
punktacja MEiN [2015 A]: 40