W dniach od 2 kwietnia do 5 kwietnia 2024 r. prowadzone będą prace związane z wdrożeniem nowej wersji systemu Repozytorium UJ. Nie będzie możliwe wprowadzanie nowych informacji do repozytorium. Za utrudnienia przepraszamy.
Fluorescent banding pattern and species-specific DNA marker in Rumex thyrsiflorus Fingerh
pl
dc.type
JournalArticle
pl
dc.description.physical
70-77
pl
dc.abstract.en
The object of this work was to analyze the karyotype structure of Rumex thyrsiflorus using differential fluorescent methods of chromosome staining (C-banding/DAPI and CMA3/DA/DAPI) and molecular sex markers. The results obtained were compared with data on the structure of the sex chromosomes and autosomes in R. acetosa, a model species in studies of sex determination and sex chromosome evolution in plants with an XX/XY1Y2 system. A high level of similarity was found in the sex chromosome structure of the 2 species, along with small differences in their autosomal complexes. It suggests that differentiation of these 2 closely related species was not accompanied by major structural changes within their sex chromosomes. Molecular tests, however, revealed differences in the composition of male-specific repetitive sequence RAYSII, occurring in the Y 1 chromosome. Amplification of this sequence showed the presence of a single product (∼700 bp) in R. acetosa and of 2 products (∼600 bp and ∼700 bp) in R. thyrsiflorus. The longer product (∼700 bp) was also revealed in R. arifolius, another species closely related to R. acetosa. The shorter DNA fragment, characteristic of R. thyrsiflorus, differed from the common product by of a large indel with a length of 110 bp. This fragment may serve as a species-specific molecular marker useful in taxonomical and population studies as well as in further research on the sex chromosome differentiation in R. thyrsiflorus.
pl
dc.subject.en
C-banding/DAPI
pl
dc.subject.en
CMA 3 /DA/DAPI
pl
dc.subject.en
male-specific sequence RAYSII
pl
dc.subject.en
molecular marker
pl
dc.subject.en
rumex acetosa
pl
dc.subject.en
rumex thyrsiflorus
pl
dc.description.volume
137
pl
dc.description.number
1
pl
dc.identifier.doi
10.1159/000339383
pl
dc.identifier.eissn
1424-859X
pl
dc.title.journal
Cytogenetic and Genome Research
pl
dc.language.container
eng
pl
dc.affiliation
Wydział Biologii i Nauk o Ziemi : Instytut Botaniki
pl
dc.subtype
Article
pl
dc.rights.original
bez licencji
pl
.pointsMNiSW
[2012 A]: 20
Pliki tej pozycji
Plik
Rozmiar
Format
Przeglądanie
Nie ma plików powiązanych z tą pozycją.
Pozycja umieszczona jest w następujących kolekcjach