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FT-IR imaging for quantitative determination of liver fat content in non-alcoholic fatty liver
In this work we apply FT-IR imaging of large areas of liver tissue cross-section samples (∼5 cm × 5 cm) for quantitative assessment of steatosis in murine model of Non-Alcoholic Fatty Liver (NAFLD). We quantified the area of liver tissue occupied by lipid droplets (LDs) by FT-IR imaging and Oil Red O (ORO) staining for comparison. Two alternative FT-IR based approaches are presented. The first, straightforward method, was based on average spectra from tissues and provided values of the fat content by using a PLS regression model and the reference method. The second one – the chemometric-based method – enabled us to determine the values of the fat content, independently of the reference method by means of k-means cluster (KMC) analysis. In summary, FT-IR images of large size liver sections may prove to be useful for quantifying liver steatosis without the need of tissue staining.
dc.abstract.en | In this work we apply FT-IR imaging of large areas of liver tissue cross-section samples (∼5 cm × 5 cm) for quantitative assessment of steatosis in murine model of Non-Alcoholic Fatty Liver (NAFLD). We quantified the area of liver tissue occupied by lipid droplets (LDs) by FT-IR imaging and Oil Red O (ORO) staining for comparison. Two alternative FT-IR based approaches are presented. The first, straightforward method, was based on average spectra from tissues and provided values of the fat content by using a PLS regression model and the reference method. The second one – the chemometric-based method – enabled us to determine the values of the fat content, independently of the reference method by means of k-means cluster (KMC) analysis. In summary, FT-IR images of large size liver sections may prove to be useful for quantifying liver steatosis without the need of tissue staining. | pl |
dc.affiliation | Wydział Chemii : Zakład Fizyki Chemicznej | pl |
dc.affiliation | Wydział Lekarski : Zakład Farmakologii Doświadczalnej | pl |
dc.affiliation | Pion Rektora : Jagiellońskie Centrum Rozwoju Leków | pl |
dc.cm.id | 73237 | |
dc.contributor.author | Kochan, Kamila - 151367 | pl |
dc.contributor.author | Maślak, Edyta - 211387 | pl |
dc.contributor.author | Chłopicki, Stefan - 128995 | pl |
dc.contributor.author | Barańska, Małgorzata - 127224 | pl |
dc.date.accessioned | 2015-12-14T12:26:17Z | |
dc.date.available | 2015-12-14T12:26:17Z | |
dc.date.issued | 2015 | pl |
dc.description.number | 15 | pl |
dc.description.physical | 4997-5002 | pl |
dc.description.volume | 140 | pl |
dc.identifier.doi | 10.1039/C5AN00737B | pl |
dc.identifier.eissn | 1364-5528 | pl |
dc.identifier.issn | 0003-2654 | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/18224 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights.licence | bez licencji | |
dc.subtype | Article | pl |
dc.title | FT-IR imaging for quantitative determination of liver fat content in non-alcoholic fatty liver | pl |
dc.title.journal | Analyst | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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