Simple view
Full metadata view
Authors
Statistics
Monocyte chemoattractant protein-induced protein 1 (MCPIP1) enhances angiogenic and cardiomyogenic potential of murine bone marrow-derived mesenchymal stem cells
The current evidence suggests that beneficial effects of mesenchymal stem cells (MSCs) toward myocardial repair are largely due to paracrine actions of several factors. Although Monocyte chemoattractant protein-induced protein 1 (MCPIP1) is involved in the regulation of inflammatory response, apoptosis and angiogenesis, whether MCPIP1 plays any role in stem cell-induced cardiac repair has never been examined. By employing retroviral (RV)-transduced overexpression of MCPIP1, we investigated the impact of MCPIP1 on viability, apoptosis, proliferation, metabolic activity, proteome, secretome and differentiation capacity of murine bone marrow (BM) - derived MSCs. MCPIP1 overexpression enhanced angiogenic and cardiac differentiation of MSCs compared with controls as indicated by elevated expression of genes accompanying angiogenesis and cardiomyogenesis in vitro. The proangiogenic activity of MCPIP1-overexpressing MSCs (MCPIP1-MSCs) was also confirmed by increased capillary-like structure formation under several culture conditions. This increase in differentiation capacity was associated with decreased proliferation of MCPIP1-MSCs when compared with controls. MCPIP1-MSCs also expressed increased levels of proteins involved in angiogenesis, autophagy, and induction of differentiation, but not adverse inflammatory agents. We conclude that MCPIP1 enhances endothelial and cardiac differentiation of MSCs. Thus, modulating MCPIP1 expression may be a novel approach useful for enhancing the immune-regulatory, anti-apoptotic, anti-inflammatory and regenerative capacity of BM-derived MSCs for myocardial repair and regeneration of ischemic tissues.
cris.lastimport.scopus | 2024-04-24T03:02:15Z | |
cris.lastimport.wos | 2024-04-09T21:12:43Z | |
dc.abstract.en | The current evidence suggests that beneficial effects of mesenchymal stem cells (MSCs) toward myocardial repair are largely due to paracrine actions of several factors. Although Monocyte chemoattractant protein-induced protein 1 (MCPIP1) is involved in the regulation of inflammatory response, apoptosis and angiogenesis, whether MCPIP1 plays any role in stem cell-induced cardiac repair has never been examined. By employing retroviral (RV)-transduced overexpression of MCPIP1, we investigated the impact of MCPIP1 on viability, apoptosis, proliferation, metabolic activity, proteome, secretome and differentiation capacity of murine bone marrow (BM) - derived MSCs. MCPIP1 overexpression enhanced angiogenic and cardiac differentiation of MSCs compared with controls as indicated by elevated expression of genes accompanying angiogenesis and cardiomyogenesis in vitro. The proangiogenic activity of MCPIP1-overexpressing MSCs (MCPIP1-MSCs) was also confirmed by increased capillary-like structure formation under several culture conditions. This increase in differentiation capacity was associated with decreased proliferation of MCPIP1-MSCs when compared with controls. MCPIP1-MSCs also expressed increased levels of proteins involved in angiogenesis, autophagy, and induction of differentiation, but not adverse inflammatory agents. We conclude that MCPIP1 enhances endothelial and cardiac differentiation of MSCs. Thus, modulating MCPIP1 expression may be a novel approach useful for enhancing the immune-regulatory, anti-apoptotic, anti-inflammatory and regenerative capacity of BM-derived MSCs for myocardial repair and regeneration of ischemic tissues. | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biologii Komórki | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Ogólnej | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej | pl |
dc.contributor.author | Łabędź-Masłowska, Anna - 108413 | pl |
dc.contributor.author | Lipert, Barbara - 175041 | pl |
dc.contributor.author | Berdecka, Dominika | pl |
dc.contributor.author | Kędracka-Krok, Sylwia - 128739 | pl |
dc.contributor.author | Jankowska, Urszula - 103969 | pl |
dc.contributor.author | Kamycka, Elżbieta - 114640 | pl |
dc.contributor.author | Sekuła, Małgorzata - 184996 | pl |
dc.contributor.author | Madeja, Zbigniew - 130173 | pl |
dc.contributor.author | Dawn, Buddhadeb | pl |
dc.contributor.author | Jura, Jolanta - 128552 | pl |
dc.contributor.author | Zuba-Surma, Ewa - 174273 | pl |
dc.date.accessioned | 2015-10-02T11:44:54Z | |
dc.date.available | 2015-10-02T11:44:54Z | |
dc.date.issued | 2015 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.admin | [AB] Berdecka, Dominika | pl |
dc.description.admin | [AB] Kamycka, Elżbieta | pl |
dc.description.number | 7 | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 10 | pl |
dc.identifier.articleid | e0133746 | pl |
dc.identifier.doi | 10.1371/journal.pone.0133746 | pl |
dc.identifier.eissn | 1932-6203 | pl |
dc.identifier.project | ROD UJ / P | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/15897 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa | * |
dc.rights.licence | CC-BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/legalcode.pl | * |
dc.share.type | otwarte czasopismo | |
dc.subtype | Article | pl |
dc.title | Monocyte chemoattractant protein-induced protein 1 (MCPIP1) enhances angiogenic and cardiomyogenic potential of murine bone marrow-derived mesenchymal stem cells | pl |
dc.title.journal | PLoS ONE | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.
Views
0
Views per month
Open Access
License
Except as otherwise noted, this item is licensed under the Attribution 4.0 International licence