W dniach od 2 kwietnia do 5 kwietnia 2024 r. prowadzone będą prace związane z wdrożeniem nowej wersji systemu Repozytorium UJ. Nie będzie możliwe wprowadzanie nowych informacji do repozytorium. Za utrudnienia przepraszamy.
Resonance Raman and EPR spectroscopy studies of untreated spring wheat leaves
autor:
Wesełucha-Birczyńska Aleksandra , Łabanowska Maria , Kurdziel Magdalena , Filek Maria
tytuł czasopisma:
Vibrational Spectroscopy
tytuł numeru:
Selected Papers from the 6th International Conference on Advanced Vibrational Spectroscopy (ICAVS-6) and Two-Dimensional Correlation Spectroscopy (2DCOS-6) Sonoma County, CA, USA, 9–17th June 2011
Leaves are the structures in plants that have evolved primarily to absorb sunlight and to convert this
energy into carbohydrates, which is the essence of the photosynthesis process. In the current study
Raman and electron paramagnetic resonance (EPR) spectroscopic methods were applied to investigate
the drought stress occurring in the fresh wheat leaves immediately after cutting them off from the
rest of the plant. Resonance Raman microspectroscopy mapping allowed us to visualize the changes
in concentrations of carotenoids, playing an important role in the photosynthetic membrane, as well
as quinones and carbohydrates in the selected areas in plant tissues. EPR spectroscopy was used
to detect free radicals in the leaf tissue directly. Besides these very reactive, short lifetime species
(monodehydroascorbate–ascorbate radical (MDA)), the long-living semiquinone and carbohydrate rad-
icals have been also detected.