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Thermal degradation of biological DNA studied by dielectric spectroscopy
Dielectric spectroscopy was tested as an alternative tool to study degradation of deoxyribonucleic acid (DNA) in its solid form. The specimens, prepared from biological DNA, were periodically heated and cooled according to a programmed scheme. Simultaneously, their dielectric parameters (permittivity and dielectric loss) were monitored as function of frequency and temperature. The analysis of Bode plots allowed to determine the upper limit of thermal stability of solid DNA at 120 °C, because heating at higher temperatures resulted in irreversible changes. These changes were identified as denaturation by gel electrophoresis and UV–vis absorption methods.
cris.lastimport.wos | 2024-04-09T21:24:30Z | |
dc.abstract.en | Dielectric spectroscopy was tested as an alternative tool to study degradation of deoxyribonucleic acid (DNA) in its solid form. The specimens, prepared from biological DNA, were periodically heated and cooled according to a programmed scheme. Simultaneously, their dielectric parameters (permittivity and dielectric loss) were monitored as function of frequency and temperature. The analysis of Bode plots allowed to determine the upper limit of thermal stability of solid DNA at 120 °C, because heating at higher temperatures resulted in irreversible changes. These changes were identified as denaturation by gel electrophoresis and UV–vis absorption methods. | pl |
dc.affiliation | Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki Molekularnej | pl |
dc.contributor.author | Nizioł, Jacek | pl |
dc.contributor.author | Ekiert, Robert - 104021 | pl |
dc.contributor.author | Kuczkowska, Justyna | pl |
dc.contributor.author | Fryń, Patryk - 190075 | pl |
dc.contributor.author | Marzec, Monika - 100126 | pl |
dc.date.accessioned | 2020-01-03T14:15:12Z | |
dc.date.available | 2020-01-03T14:15:12Z | |
dc.date.issued | 2019 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 80 | pl |
dc.identifier.articleid | 106158 | pl |
dc.identifier.doi | 10.1016/j.polymertesting.2019.106158 | pl |
dc.identifier.eissn | 1873-2348 | pl |
dc.identifier.issn | 0142-9418 | pl |
dc.identifier.project | ROD UJ / OP | pl |
dc.identifier.uri | https://ruj.uj.edu.pl/xmlui/handle/item/129992 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa | * |
dc.rights.licence | CC-BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/legalcode.pl | * |
dc.share.type | inne | |
dc.subtype | Article | pl |
dc.title | Thermal degradation of biological DNA studied by dielectric spectroscopy | pl |
dc.title.journal | Polymer Testing | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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Except as otherwise noted, this item is licensed under the Attribution 4.0 International licence