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Plasma-assisted nanoscale protein patterning on Si substrates via colloidal lithography
Selective immobilization of proteins in well-defined patterns on substrates has recently attracted considerable attention as an enabling technology for applications ranging from biosensors and BioMEMS to tissue engineering. In this work, a method is reported for low-cost, large scale and high throughput, selective immobilization of proteins on nanopatterned Si, based on colloidal lithography and plasma processing to define the areas( < 300 nm) where proteins are selectively immobilized. A close-packed monolayer of PS microparticles is deposited on oxidized Si and, either after microparticle size reduction or alternatively after metal deposition through the PS close-packed monolayer, is used as etching mask to define
cris.lastimport.scopus | 2024-04-07T17:14:25Z | |
dc.abstract.en | Selective immobilization of proteins in well-defined patterns on substrates has recently attracted considerable attention as an enabling technology for applications ranging from biosensors and BioMEMS to tissue engineering. In this work, a method is reported for low-cost, large scale and high throughput, selective immobilization of proteins on nanopatterned Si, based on colloidal lithography and plasma processing to define the areas( < 300 nm) where proteins are selectively immobilized. A close-packed monolayer of PS microparticles is deposited on oxidized Si and, either after microparticle size reduction or alternatively after metal deposition through the PS close-packed monolayer, is used as etching mask to define $SiO_{2}$ nanoislands (on Si). $C_{4} F_{8}$ plasma was used to selectively etch and modify the $SiO_{2}$ nanoislands while depositing a fluorocarbon layer on the Si surface. The plasma-treated surfaces were chemically characterized in terms of functional group identification through XPS analysis and reaction with specific molecules. Highly selective protein immobilization mainly through physical adsorption on $SiO_{2}$ nanoislands and not on surrounding Si was observed after $C_{4} F_{8}$ plasma-induced chemical modification of the substrate. The thickness of the immobilized protein monolayer was estimated by means of AFM image analysis. The method reported herein constitutes a cost-efficient route toward rapid, large surface, and high-density patterning of biomolecules on solid supports that can be easily applied in BioMEMS or microanalytical systems. | pl |
dc.affiliation | Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego | pl |
dc.contributor.author | Malainou, A. | pl |
dc.contributor.author | Tsougeni, K. | pl |
dc.contributor.author | Ellinas, K. | pl |
dc.contributor.author | Petrou, P. S. | pl |
dc.contributor.author | Constantoudis, V. | pl |
dc.contributor.author | Sarantopoulou, E. | pl |
dc.contributor.author | Awsiuk, Kamil - 103592 | pl |
dc.contributor.author | Bernasik, A. | pl |
dc.contributor.author | Budkowski, Andrzej - 127461 | pl |
dc.contributor.author | Markou, A. | pl |
dc.contributor.author | Panagiotopoulos, I. | pl |
dc.contributor.author | Kakabakos, S. E. | pl |
dc.contributor.author | Gogolides, E. | pl |
dc.contributor.author | Tserepi, A. | pl |
dc.date.accessioned | 2015-07-01T10:58:59Z | |
dc.date.available | 2015-07-01T10:58:59Z | |
dc.date.issued | 2013 | pl |
dc.description.number | 50 | pl |
dc.description.physical | 13743-13751 | pl |
dc.description.publication | 0,5 | pl |
dc.description.volume | 117 | pl |
dc.identifier.doi | 10.1021/jp407810x | pl |
dc.identifier.eissn | 1520-5215 | pl |
dc.identifier.issn | 1089-5639 | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/11016 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Dodaję tylko opis bibliograficzny | * |
dc.rights.licence | bez licencji | |
dc.rights.uri | * | |
dc.subtype | Article | pl |
dc.title | Plasma-assisted nanoscale protein patterning on Si substrates via colloidal lithography | pl |
dc.title.journal | The Journal of Physical Chemistry. A | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |