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FTIR spectroscopy is a widely used technique that provides insights into disease processes at the molecular
level. Due to its numerous advantages it is becoming an increasingly powerful tool for the study of biologi-
cal materials and has the potential to become an excellent diagnostic method, especially considering the
low cost of trans
fl
ection substrates. However, questions about the usefulness of the trans
fl
ection measure-
ment mode due to the complicated nature of physical processes occurring during the measurement and in
particular the Electric Field Standing Wave (EFSW) e
ff
ect have been raised. In this paper we present a com-
parison of the two most common FT-IR measurement modes: transmission and transfection using healthy
and pathologically altered tissue (histiocytic sarcoma). We found that the major di
ff
erences between normal
and cancerous tissue were associated with changes DNA and carbohydrate content. In particular we ident-
i
fi
ed a band at 964 cm
−
1
assigned to a nucleic acid phosphodiester backbone mode, which appeared more
pronounced in cancerous tissue irrespective of the substrate. We applied Principal Component Analysis,
Unsupervised Hierarchical Cluster Analysis and
k
-means clustering to transmission and trans
fl
ection sub-
strates and found that both measurement modes were equally capable of discrimination normal form can-
cerous tissue. Moreover, the di
ff
erences between spectra from cancerous and normal tissue were
signi
fi
cantly more important than the ones arising from the measurement modes.
wydział: instytut / zakład / katedra:
Wydział Chemii : Zakład Fizyki Chemicznej
typ:
artykuł w czasopiśmie
podtyp:
artykuł
punktacja MEiN [2015 A]: 40
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